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Ascidiella aspersa (European sea squirt) has a lifespan of 18 months, from mid-summer time to the following year’s winter (Millar, 1952). In early to mid-summer larval settlement and metamorphosis occurs and takes approximately 24 hours at 20°C (NIMPIS, 2010). Progress is continued through the fall and into the winter, although development in winter is decreased. Upon reaching a dimension of 30mm, the male sexual organs start to fill with sperm and at a much greater size the female eggs start to fill the oviducts. At a measurement of 40mm, each sex organs are absolutely mature (Millar, 1952). One spawning season happens, normally one year after larval settlement (Millar, 1952). Beneath laboratory circumstances (Bolton, 1996), there was an noticed synchronous response to gentle in the spawning of ascidians.

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